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. 2019 Oct 10;8(10):1231. doi: 10.3390/cells8101231

Figure 2.

Figure 2

XANES traces showing the effect of 1 week’s incubation on metal oxidation states. (a) Aβ and (b) α-syn on the oxidation state of Fe, and (c) Aβ and (d) α-syn on the oxidation state of Cu. Ferric and ferrous standards (Fe(III)Cl3 and [Fe(II) + 1,10-phenanthroline], respectively) were used for linear combination fitting (LCF) of the spectra in (a) and (b), and cupric and cuprous standards (Cu(II)SO4) and [Cu(I)-glutathione] were used for (c) and (d); the results from the LCF are tabulated in each panel; below the experimentally-acquired XANES spectra are the reference standards used to fit them for each time point (a)–(d). In (a) and (b), inclusion of a small contribution from iron(III) ammonium citrate in the LCF resulted in improved quality of fit. χ2 values are as follows for t0 and t1wk fits, respectively: (a) 0.06, 0.03; (b) 0.04, 0.02; (c) 0.07, 0.06; (d) 0.002, 0.04. Traces for metal-peptide incubations are vertically offset from the reference standards for clarity.