Figure 2.

RNA expression in isolated human posterior limbus tissue. (A) Microdissection of posterior limbus into trabecular meshwork (TM), transition zone (TZ) and peripheral endothelium (PE) tissues for total RNA extraction. Target gene expression in various tissues compared to central corneal endothelium (CE). Data are presented as mean expression fold changes and SD. (B) Neural crest markers (Snail1, Nestin, Sox9). (C) POM markers (Pitx2, FoxC1, p75^NTR). (D) Corneal endothelial differentiation marker (ZO1, Slc4a11, Col8a2). (E) Pluripotency markers (Oct3/4, c-Myc, Klf4, ABCG2, Nanog). * p < 0.05, ** p < 0.01, when compared to CE (Mann–Whitney U test).