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Primary culture of porcine transition zone (TZ) cells. (A) Dissected TZ tissue with minimal TM and PE. (B,C) Primary TZ cells in culture with TZ stem cell medium (TZSCM) for 4 and 10 days, respectively. (D–F) Primary TZ cells in proliferative M4 medium at day 2, 7 and 14 for monolayer formation. (G,H) Cells in endothelial stabilizing M5 medium for 7 days. A homogenous monolayer of tightly-packed polygonal to hexagonal-like cells was generated. Scale bars: 50 μm.
