Skip to main content
. 2019 Oct 14;8(10):1253. doi: 10.3390/cells8101253

Figure 1.

Figure 1

Figure 1

In order to identify genes regulated by I942, confluent human umbilical vein endothelial cells HUVECs were stimulated for 48 h with (a) 10 μM forskolin plus 10 μM rolipram (F/R) or (b) 100 μM I942. Total RNA was then extracted from cells and processed for RNA-SEQ and plotted as volcano plots as described in Materials and Methods, with gene expression changes greater than 2-fold being indicated in red. Genes that were significantly (p < 0.05, dotted blue line) regulated individually or by both treatments (see Supplementary Materials) are expressed as a Venn diagram in (c).