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. 2019 Oct 14;8(10):1253. doi: 10.3390/cells8101253

Figure 8.

Figure 8

Confluent HUVECs were pre-incubated with siRNA to c-Jun or non-targeting siRNA (NT) for 24 h and then stimulated for 5 h in the presence or absence of 100 μM I942 (upper left panel) or 10 μM F/R (upper right panel). Cell extracts were then prepared and immunoblotted with antibodies to SOCS3 protein, c-Jun and GAPDH, as a loading control. Densitometry was carried out on 3 Western blots and results are shown as a bar graph. Significant increases in SOCS3 protein expression, relative to diluent-stimulated control cells, are indicated; *** p < 0.001. Significant inhibition of SOCS3 induction relative to NT siRNA-treated cells is also indicated; ### p < 0.001.