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. 2019 Oct 14;8(10):1253. doi: 10.3390/cells8101253

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) HUVECs were stimulated for the indicated times with IL6 (5 ng/mL) plus sIL6Rα (25 ng/mL) in the presence or absence of 100 μM I942. Cell extracts were then immunoblotted with antibodies to pERK/ERK, pAKT/AKT, ICAM1 or GAPDH, as indicated. (b) Densitometric values from 3 separate immunoblots are shown on the left with significant decreases in the ratio of pERK/ERK and pAKT/AKT in cells stimulated with IL6 and I942, relative to stimulation with IL6 alone, being indicated; # p < 0.05 (n = 3).