Figure 3.

Non–breast cancer cells and TP53 mutant cells that survive doxorubicin treatment engulf neighbors. (A–E) The indicated GFP- or mCherry-expressing cells were co-plated with cells of the opposite color as in Fig. 2 E that were either NT or doxorubicin treated (DOXO). After 7 d, images of engulfed cells were captured (left) and random fields from triplicate wells were quantitated for engulfment (graphs, right). More than 100 treated cells were scored for engulfment in each well and percentages determined. Error bars represent SEM. (F) mCherry-expressing MCF-7 cells with both alleles of TP53 edited by CRISPR-Cas9 were doxorubicin treated as in A and co-plated with GFP-expressing MCF-7 cells. After 7 d, images were captured of representative remaining TP53 edited cells that had engulfed.