Figure 1.

Characterization of EPCs

(A) Scheme of iPSCs differentiation into BCLECs. iPSCs were first differentiated during 10 days into EPCs (CD31⁺ cells). These cells were then isolated by MACS and specified/expanded into BCLECs for four passages.

(B) Expression of BCEC markers was assessed by flow cytometry on isolated CD31⁺ cells. Percentages of positive cells were calculated based on the isotype controls (1%; dark blue scatter plot).

(C) Expression of BCEC markers on CD31⁺ cells by immunocytochemistry. Scale bar corresponds to 50 μm.