Figure 3.
Exogenous ALDH1A1 Enhances the Formation and Proliferation of Spheroids and Tumorigenicity (EMN24 Cells)
(A) Western blot analyses of spheroid cells after infection with the indicated lentiviruses.
(B) ALDH activity in the infected cells after ALDEFLUOR staining.
(C) Time course of proliferation of infected cells. n = 4 independent experiments, p < 0.01, Student's t test.
(D) Bright-phase images of the infected cells. Scale bars, 100 μm.
(E) Volumes (mean ± SEM) of xenograft tumors from 1 × 105 infected cells. n = 6 independent experiments, p < 0.01, Student's t tests. Images of whole resected tumor xenograft tumors excised on day 25 (bottom). Scale bar, 10 mm.
(F) H&E staining and immunostaining of xenograft tumors, 25 days after transplantation. Scale bars, 100 μm.
∗∗p < 0.01. See also Figure S2.