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. 2019 Sep 12;13(4):627–641. doi: 10.1016/j.stemcr.2019.08.007

Figure 2.

Figure 2

Establishment of Endoderm-Derived Hepatic Organoids

(A) Organoid formation potency of sorted EpCAM+ and EpCAM− cells and bright-field images of organoid culture from various passages, where P indicates passage number.

(B) Phenotypic characterization of endoderm-derived WT organoid culture at different passage numbers (p6, p21, and p48) during expansion on EM.

(C) Confocal images of organoids for EpCAM, CK19, and HNF4α. Nuclei were co-stained with DAPI. In the dataset for p10 organoids, EpCAM∖HNF4α and ZO-1 were stained in the whole-mount organoids. The others were stained from frozen sections.

(D) Immunohistochemical staining of CK18 and AFP in organoids. H&E indicates hematoxylin-eosin staining.

(E) GSEA plots for differentially expressed genes during iPSC to endoderm induction and endoderm to organoid differentiation. NES and FDR q values are listed for each gene set analyzed.