Figure 4.

In Vitro and In Vivo Functionality of Endoderm-Derived Hepatic Organoids

(A) Albumin secretion of the WT organoids from different passage numbers (p6, p23, p48) cultured in EM and DM conditions as measured by ELISA. Data are shown as mean ± SD of n = 3, given as ngALB/day/million cells.

(B) CYP3A4 activity in organoids cultured in EM and DM conditions expressed as RLU/mL/million cells.

(C) Uptake of low-density lipoprotein (LDL) detected on day 14 by immunofluorescence staining in WT DM organoids from p10 and p48.

(D) Glycogen storage function of WT DM organoids from p10 and p48 by periodic-acid-Schiff (PAS) staining (magenta). EM condition was used as undifferentiated control.

(E) Immunohistochemistry with anti-GFP and anti-human albumin antibodies in DMN-treated NSG mouse liver sections transplanted with mature eHEPOs. The presence of GFP⁺ and human ALB⁺ cells demonstrates engraftment of hepatocytes into mouse liver.

Error bars in (A) and (B) denote ±SD of three independent experiments (^∗p ≤ 0.05; ^∗∗p ≤ 0.01; ^∗∗∗p ≤ 0.001).