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. 2019 Sep 19;13(4):700–712. doi: 10.1016/j.stemcr.2019.08.010

Figure 1.

Figure 1

Early Cell Progeny of Postnatal NPCs

(A) (a) UbC-StarTrack clonal mixture includes 12 ubiquitous plasmids expressing six different fluorescent reporter proteins (XFPs) either in cytoplasm or in the nucleus (H2B). Co-electroporation with hyperactive PiggyBac transposase (hyPBase) and Cre-recombinase (Cre-ERT2) is required. (b) Postnatal electroporation was performed at P1. Tamoxifen administration 1 day after electroporation activated Cre-recombinase and eliminated non-integrated constructs. Brains were analyzed at 3 days (P4) and 7 days (P7) after electroporation.

(B) UbC-StarTrack-labeled NPCs express BLBP 3 days after electroporation. Scale bars, 25 μm.

(C) EGFR labels active NPCs as well as TAPs. Two clones containing pairs of sibling cells (arrowheads and stars) are shown 3 days after electroporation. Scale bars, 25 μm.

(D) Sibling astrocytes dispersed through the cortical layers 6 days after electroporation (P7). Labeled cells scattered into the cortex and other ventricular adjacent areas, displaying differentiated morphologies. The color codes are detailed in (a) to (c). Scale bars, 50 μm (D) and 25 μm (Da, Db, Dc).