Figure 1.

Small-Molecule Screen for Feeder-free Maintenance of Naive hESCs

(A) Schematic of high-throughput screen performed to identify compounds supporting feeder-free culture of naive hESCs. Dot plot presents mean Z scores for LTR7Y-zsGreen intensity results from the screen. The gray line indicates a cutoff of Z scores ≥2. Small molecules achieving this cutoff in at least two replicates were considered as hits (blue). Other samples (orange) and DMSO controls (red) did not pass this cutoff. A full list of scores is given in Table S1.

(B) Summary table with hits from the small-molecule screen. Asterisks denote compounds targeting pathways not previously demonstrated to play a role in establishment/maintenance of naive pluripotency.

(C) Representative images of LTR7Y-zsGreen cells after treatment with small-molecule hits. Scale bars, 50 μm.

(D) Fluorescence-activated cell sorting (FACS) quantification of LTR7Y-zsGreen signal after treatment with dasatinib, crenolanib, and AZD5438.