Fig. 5.

Integrin β3 was involved in IL-8/CXCR1/2-induced invasion of HCC cells. a Real-time PCR and Western blot analysis of integrin β3 expression in Huh-7 and HepG2 cells after incubation with different concentrations of IL-8 for 24 h. b Huh-7 and HepG2 cells were transfected with integrin β3 siRNA (si-β3) or control siRNA (C siRNA) for 48 h, and in vitro invasion was measured with Transwell assay in the presence or absence of IL-8 (100 ng/ml) for 24 h. c HCCLM3 cells were transfected using IL-8 siRNA with or without integrin β3 cDNA, and in vitro invasion was measured with Transwell assay. d Real-time PCR and Western blot analysis of integrin β3 expression in Huh-7 and HepG2 cells after transfection of CXCR1 siRNA, CXCR2 siRNA or C siRNA followed by stimulation with IL-8 (100 ng/ml) for 24 h. Results are expressed as the mean ± SEM. *P < 0.05 compared with the control group. #P < 0.05 compared with the IL-8-treated group. ※P < 0.05 compared with the IL-8 siRNA-treated group