Fig. 6.

PI3K/Akt signaling pathway was involved in IL-8 mediated HCC cell invasion and integrin β3 upregulation. a Western blot analysis of total and phosphorylated PI3K and Akt in C siRNA and IL-8 siRNA transfected cells. b After pretreatment with PI3K-specific inhibitor LY294002 (25 μM) for 30 min, cells were stimulated with IL-8 (100 ng/ml) for 24 h, and in vitro invasion was measured with Transwell assay. c After pretreatment with LY294002 (25 μM) for 30 min, cells were stimulated with IL-8 (100 ng/ml) for 24 h, and expression of integrin β3, PI3K, p-PI3K, Akt and p-Akt was measured by western blot. Results are expressed as the mean ± SEM. *P < 0.05 compared with the control group. #P < 0.05 compared with the IL-8-treated group