Table 1.
mRNA | Primer sequence | Product size (bp) | Ta (°C) |
---|---|---|---|
β-actin |
F: TCATGAAGTGTGACGTTGACATCC R: GACTGTTACTGAGCTGCGTTTTAC |
311 | 53 |
TNF-α |
F: ACTGAACTTCGGGGTGATTG R: GTCGTAGCAAACCACCAAGC |
153 | 56 |
IL-6 |
F: ATATGTTCTCAGGGAGATCTTGGAA R: GTGCATCATCGCTGTTCATACA |
80 | 60 |
GPAT-1 |
F: AGACACAGGCAGGGAATCCAC R: AATTCCCGGAGAAGCCCAG |
103 | 56 |
ACC |
F: GCCCACTTTCTTCTATCACGCTAA R: GAAGACGGCAGCATGAACTG |
159 | 56 |
SREBP-1c |
F: CAGAGGGACTACAGGCTGAGAAAG R: CACGTAGATCTCTGCCAGTGTTG |
204 | 56 |
* NCBI Reference Sequence, bp indicated base pair