Skip to main content
. 2019 Nov 4;19:294. doi: 10.1186/s12906-019-2716-0

Table 1.

Primer sequences and conditions for qRT-PCR

mRNA Primer sequence Product size (bp) Ta (°C)
β-actin

F: TCATGAAGTGTGACGTTGACATCC

R: GACTGTTACTGAGCTGCGTTTTAC

* NM_031144.3

311 53
TNF-α

F: ACTGAACTTCGGGGTGATTG

R: GTCGTAGCAAACCACCAAGC

* HQ201305.1

153 56
IL-6

F: ATATGTTCTCAGGGAGATCTTGGAA

R: GTGCATCATCGCTGTTCATACA

*NM_031512

80 60
GPAT-1

F: AGACACAGGCAGGGAATCCAC

R: AATTCCCGGAGAAGCCCAG

*AF021348

103 56
ACC

F: GCCCACTTTCTTCTATCACGCTAA

R: GAAGACGGCAGCATGAACTG

*XM_017598248.1

159 56
SREBP-1c

F: CAGAGGGACTACAGGCTGAGAAAG

R: CACGTAGATCTCTGCCAGTGTTG

*NM_001276708.1

204 56

* NCBI Reference Sequence, bp indicated base pair