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. 2019 Nov 4;19:1037. doi: 10.1186/s12885-019-6251-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — SMAD4 Y353C reduces the expression of E-cadherin but promotes that of Vimentin. a, b Western blot analysis of EMT markers was performed in the SW1990 and Panc-1 cell lines. GAPDH was included as a loading control. c The relative mRNA levels of EMT markers were examined by qRT-PCR. All statistical data were analysed by one-way ANOVA. Three independent experiments were conducted for each assay (*P < 0.05 vs SMAD4 wt)