Fig. 5.

Influence of serum free culture conditions on the paracrine signature of explant-derived cells (EDCs). a Select cytokines from an unbiased protein array profiling cytokine content within EDC conditioned media under hypoxic (1% oxygen) basal media conditions. *p < 0.05 vs. media conditioned by EDCs cultured within standard (STD) cell conditions. n=4 per group. b Select cytokines from an unbiased proteome array showing differential expression within media conditioned by STD EDCs and exSF Phys Env EDCs. *p < 0.05 vs. media conditioned by EDCs cultured within standard (STD) cell conditions. n=4 per group. c Effect of cell culture conditions on extracellular vesicle concentration and size. *p < 0.05 vs. all. N=3-4 per group. d Up and downregulated miRNAs in EVs isolated from exSF Phys Env EDCs compared to serum STD EDCs. n=3. FGF basic: Fibroblast growth factor basic; Flt-3: tyrosine kinase 3; GM-CSF: Granulocyte-macrophage colony-stimulating factor; HGF: Hepatocyte growth factor; ICAM: Intercellular adhesion molecule; IGFBP: Insulin-like growth factor binding protein; IL: interleukin; OPG: Osteoprotegerin; PDGF: Platelet-derived growth factor; SDF-1α: stromal cell-derived factor 1 alpha; uPAR: urokinase-type plasminogen activator; VEGF: Vascular endothelial growth hormone