Fig. 6.

Detail of genomic sequence of intron1 (blue letter) and exon2 (black) Z-ISO isolated from ‘Navelate’ and displaying the T insertion in exon2 identified in ‘Pinalate’ mutant (a). The underlined sequence in exon2 is deleted as a result of abnormal splicing in a ‘Pinalate’ transcript variant. (b) Structures of Z-ISO transcripts isolated from ‘Navelate’ and ‘Pinalate’. Z-ISO WT transcript encodes a 374 amino acid protein and was isolated from ‘Navelate’ and ‘Pinalate’ fruits; Z-ISO P5 and P6 sequences were isolated from ‘Pinalate’. The Z-ISO P6 contains the T insertion in exon 2 (position 1587_1588) and Z-ISO P5 transcript has a 42 bp deletion at the junction of intron1-exon2 plus T insertion in exon 2 (position 1587_1588). Alterations in P5 and P6 transcripts sequences resulted in premature stop codons and generated truncated proteins. Tp, Predicted plastid transit peptide