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. 2019 Oct 17;20(20):5156. doi: 10.3390/ijms20205156

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Microcalorimetric binding studies of metal oxanions and quaternary amines to different solute binding proteins of P. aeruginosa. (A) Titration of 40 µM PA1863 with 1 mM solutions of Na₂MoO₄, K₂Cr₂O₇, or Na₂WO₄ (6.4 µL aliquots). (B) Titration of 169 µM PA3889 with 5 mM glycine-betaine (6.4 µL aliquots) and 40 µM PA3889 with 1 mM choline (6.4 µL aliquots). Upper panel: Raw titration data. Lower panel: Dilution heat corrected and concentration normalized integrated raw data. The line is the best fit using the ‘One Binding Site’ model of the MicroCal version of ORIGIN.