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. 2019 Oct 16;8(10):1261. doi: 10.3390/cells8101261

Figure 1.

Figure 1

Niclosamide prevents the increase of S100A4, gp91phox, and paxillin in LPS- and TNFα stimulated primary microglia. (A) Primary microglia were treated with 1 μg/mL LPS or 50 ng/mL TNFα for 24, 48, or 72 h or non-treated (ctrl). Protein lysates were analyzed by western blot using anti-S100A4, anti-gp91phox, anti-paxillin, and anti-GAPDH antibodies. Primary microglia were pre-treated with DMSO (ctrl) or 10 nM, 50 nM, 100 nM niclosamide (nic). After 24 h, cells were stimulated with 1 μg/mL LPS (B) or 50 ng/mL TNFα (C) for 48 h. Protein lysates were analyzed by western blot using anti-S100A4, anti-gp91phox, anti-paxillin, and anti-GAPDH antibodies. Quantification of protein bands normalized to GAPDH and relative to control is expressed in an arbitrary unit (AU). Data are reported as mean ± S.E.M. (n = 3 independent experiments). Statistical significance was calculated by ANOVA followed by Post Hoc Tukey’s test. * p < 0.05 relative to control (A–C), # p < 0.05 relative to LPS (B) or TNFα (C).