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. 2019 Oct 19;20(20):5188. doi: 10.3390/ijms20205188

Figure 8.

Figure 8

Representative sections of aorta of Tff3−/− mice on HS used for histomorphological analysis. (A) Orcein-stained aorta with elastic fibers stained black to brown. Objective 10×, scale bar 500 μm. (B) Picrosirius red-stained aorta showing amount of collagen (red). Objective 10×, scale bar 500 μm. (C) Color-coded segmented mask from orcein-stained section. Elastic fibers are shown in black, tunica media in red, tunica adventitia in pink, and lumen area in green. (D) Color-coded segmented mask from picrosirius red-stained section. Collagen is shown in black, tunica media in red, and lumen area in green.