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. 2019 Oct 7;8(10):1210. doi: 10.3390/cells8101210

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Liver integrity was preserved by fibrocyte ablation. (a) Fibrocyte ablation caused a considerable reduction in serum levels of alanine amino transferase (ALT). Median is depicted, Mann-Whitney U test was applied. (b) Quantitative real-time PCR showed an unchanged expression of Fasl. (c) Bcl-2 western blot and optical densitometry thereof hinted at a reduced expression of Bcl-2 in the fibrocyte-ablated group. Arbitrary unit. SC n = 5; Ctrl, FC-Abl. n = 6. (d) qPCR displayed an unchanged Bcl2 expression. (e,f) Bax is expressed comparably on a protein and transcriptional level in the control- and fibrocyte-ablated group. Arbitrary unit; SC n = 4; Ctrl n = 10; FC-Abl. n = 9; All western blot experiments were performed ≥2 times, representative blots are shown. Columns and error bars depict Mean + SEM.