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. 2019 Oct 22;20(20):5241. doi: 10.3390/ijms20205241

Figure 2.

Figure 2

The onac096-1 mutant exhibits delayed natural leaf senescence. The rice plants were grown in the field under natural long-day conditions (≥14 h light/d). (a–c) Phenotypes of WT and onac096-1 (n096-1) plants at 0 DAH (a) and 48 DAH (b). Bars = 20 cm. (c) Senescing flag leaves of WT (left) and onac096-1 (right) plants at 48 DAH. The photographs are representative of five independent plants. (d–f) Changes in photosynthetic proteins (d), SPAD values (e), and PSII activity (Fv/Fm) (f) in flag leaves of WT and onac096-1 plants after heading. SPAD values (d) and Fv/Fm ratios (f) were measured in the middle part of a leaf blade every 8 d from 0 to 48 DAH. (e) Immunoblotting performed using antibodies against photosynthetic proteins (Lhca1, Lhcb1, PsaA, and PsbD). Mean and standard deviations were obtained from 10 biological repeats (d,f). Asterisks indicate statistically significant differences between onac096-1 and WT, as determined by Student’s t-test (*p < 0.05, **p < 0.01, and ***p < 0.001). The experiment was repeated twice with similar results.