Figure 6.

Altered expression of chlorophyll degradation genes (CDGs) and senescence-associated genes (SAGs) in onac096-1 during natural senescence. Total RNA was isolated from flag leaves of the WT and onac096-1 (n096-1) at 0 and 48 DAH. The transcript levels of CDGs (a–d) and SAGs (e–h) were determined by RT-qPCR and normalized to that of OsUBQ5. Relative expression was calculated using the ΔΔC_T method. Mean and SD values were obtained from three biological repeats. Asterisks indicate statistically significant differences between onac096-1 and the WT at 48 DAH according to Student’s t-test (*p < 0.05 and **p < 0.01). The experiments were repeated twice with similar results.