Table 1.
Markers employed to characterize primary BAEC and bovine fibroblasts by flow cytometry
| Marker | Location | Primary antibody | Secondary antibody | Labelled cella | References | |||
|---|---|---|---|---|---|---|---|---|
| Clone | Isotype | Dilution | Isotype (Alexa fluor 488) | Dilution | ||||
| Vimentin | Intracellular | LN-6d | Mouse monoclonal IgM | 1:100 | Anti Mouse IgG | 1:500 | ECs/Fb | [39, 42] |
| Cytokeratin | Intracellular | C-11d | Mouse monoclonal IgG1 | 1:100 | Anti Mouse IgG | 1:500 | ECs/Fb | [43–45] |
| CD34b** | Surface | NAe | Rabbit polyclonal IgG | 1:100 | Anti Rabbit IgG | 1:500 | ECs | [35] |
| CD44 | Surface | IM7f | Rat monoclonal IgG2b | 1:50 | Anti Rat IgG | 1:500 | ECs/Fb | [36, 38] |
| CD31 | Surface | CO.3E-1D4g | Mouse monoclonal IgG2a-APCc | 1:50 | – | – | ECs | [23, 35] |
aECs: Endothelial cells / Fb: Fibroblasts
bCD34: marker for hematopoietic stem cells [35]
cCD31 conjugated to Allophycocyanin (APC) (direct staining)
dSupplied by Sigma-Aldrich
eSupplied by Biorbyt
fSupplied by Bio-Rad
gSupplied by NovusBio