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. 2019 Nov 4;18:191. doi: 10.1186/s12944-019-1136-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Paradoxical roles of AMPK and SREBP-1c in NS5A-induced hepatic steatosis. a qRT-PCR analysis of the mRNA levels of FASN and ACC1 in Hepa1–6 cells co-transfected with NS5A and SREBP-1c. b and c The expression levels of SREBP-1c, ACC1 and FASN were analyzed by Western blotting in Hepa1–6 and HepG2 cells after co-transfection with NS5A and SREBP-1c. d and e The accumulation of lipid droplets in HepG2 cells was visualized by Oil Red O staining. Oil Red O extracted with isopropanol was measured at OD520. The Data are representative of three independent experiments. NS (no significance), *P < 0.05, **P < 0.01, ***P < 0.001