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Two-photon induced fluorescence spectra of Pacific Blue-labeled HSA (L = 1.5) in the presence (—) and absence (– –) of small silver colloids. The front-face excitation at 780 nm was from the femtosecond Ti : Sapphire laser. The laser beam was collimated to about 200 μm in diameter. Also are shown respective backgrounds for samples with (- - -) and without (⋯) colloids.
