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CCCP-induced degradation of MFN2 in R94Q cells. (A) Cells were treated with the uncoupler CCCP for the indicated time and immunoblotted against MFN2 in (A) and against TOM20 or VDAC1 in (B). Actin served as loading control. Size is indicated. Statistical variation is shown in (A) as Tukey boxplots and significance calculated using one-way ANOVA and Tukey’s multiple comparisons test, * p < 0.05 with n = 5 independent experiments and in (B) as individual data points, mean ± SEM.
