Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Seeding procedure of primary respiratory cells in cellulose for upside-down cell culture in Transwell chambers. Primary cells (NHBE/SAE) are mixed in GrowDex® as described below and then transferred to an inverted Transwell chamber fixed with tape in a 6-well plate. Cells are allowed to adhere overnight, and the next day, the Transwell chamber is flipped into a 24-Transwell plate. Cells are cultured submerged for 3 days and then transferred into ALI during the differentiation process.
