Figure 2.

Capsaicin suppresses tNOX expression through proteasome-mediated degradation. Aliquots of cell lysates were separated by SDS-PAGE and analyzed by Western blotting. β-Actin was detected as an internal control. Representative images are shown. Values (mean ± S. E.) are from at least three independent experiments (* p < 0.05, *** p < 0.001 for capsaicin-treated cells vs. controls). (A) tNOX expression was significantly decreased by capsaicin at 100 and 200 μM. (B) Capsaicin (200 μM) clearly reduces tNOX stability as assessed with a cycloheximide-chase assay. (C) The proteasome inhibitor, MG132, reverses the capsaicin-induced suppression of tNOX expression.