Table 2.
Stability rankings of tested RGs.
| Geomean | DeltaCt | BestKeeper | NormFinder | geNorm | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ALL | ACTB | 1.32 | ACTB | 1.08 | GAPDH | 0.29 | ACTB | 0.159 | ACTB GAPDH | 0.318 |
| GAPDH | 1.41 | GAPDH | 1.08 | HPRT1 | 0.34 | GAPDH | 0.159 | |||
| HPRT1 | 2.71 | HPRT1 | 1.16 | ACTB | 0.37 | HPRT1 | 0.265 | HPRT1 | 0.468 | |
| B2M | 4.23 | B2M | 2.14 | RPLP0 | 1.51 | B2M | 2.062 | B2M | 1.097 | |
| RPLP0 | 4.73 | RPLP0 | 2.17 | B2M | 1.55 | RPLP0 | 2.099 | RPLP0 | 1.527 | |
| NAIVE | GAPDH | 1 | GAPDH | 0.3 | GAPDH | 0.11 | GAPDH | 0.095 | ACTB GAPDH | 0.149 |
| ACTB | 1.68 | ACTB | 0.33 | ACTB | 0.16 | ACTB | 0.185 | |||
| RPLP0 | 3.22 | RPLP0 | 0.39 | RPLP0 | 0.2 | RPLP0 | 0.272 | HPRT1 | 0.254 | |
| HPRT1 | 3.94 | HPRT1 | 0.4 | B2M | 0.25 | HPRT1 | 0.322 | RPLP0 | 0.32 | |
| B2M | 4.73 | B2M | 0.46 | HPRT1 | 0.25 | B2M | 0.404 | B2M | 0.377 | |
| IFNγ + TNFα | HPRT1 | 1.32 | HPRT1 | 0.31 | RPLP0 | 0.23 | HPRT1 | 0.099 | GAPDH HPRT1 | 0.164 |
| GAPDH | 2 | GAPDH | 0.34 | B2M | 0.25 | GAPDH | 0.213 | |||
| RPLP0 | 2.45 | RPLP0 | 0.37 | HPRT1 | 0.26 | RPLP0 | 0.244 | ACTB | 0.277 | |
| ACTB | 3.94 | ACTB | 0.42 | GAPDH | 0.3 | ACTB | 0.348 | RPLP0 | 0.333 | |
| B2M | 3.98 | B2M | 0.45 | ACTB | 0.45 | B2M | 0.393 | B2M | 0.381 | |
| CTGF | RPLP0 | 1.19 | RPLP0 | 0.47 | RPLP0 | 0.21 | ACTB | 0.128 | ACTB RPLP0 | 0.278 |
| ACTB | 1.41 | ACTB | 0.48 | ACTB | 0.39 | RPLP0 | 0.139 | |||
| GAPDH | 3 | GAPDH | 0.55 | GAPDH | 0.42 | GAPDH | 0.385 | GAPDH | 0.324 | |
| B2M | 4 | B2M | 0.66 | B2M | 0.49 | B2M | 0.53 | B2M | 0.45 | |
| HPRT1 | 5 | HPRT1 | 0.81 | HPRT1 | 0.53 | HPRT1 | 0.748 | HPRT1 | 0.594 | |
| LD | ACTB | 1.32 | ACTB | 1.08 | HPRT1 | 0.24 | ACTB | 0.164 | ACTB GAPDH | 0.328 |
| GAPDH | 1.68 | GAPDH | 1.12 | GAPDH | 0.38 | GAPDH | 0.164 | |||
| HPRT1 | 2.28 | HPRT1 | 1.16 | ACTB | 0.39 | HPRT1 | 0.227 | HPRT1 | 0.466 | |
| RPLP0 | 4.23 | RPLP0 | 2.17 | B2M | 1.5 | RPLP0 | 2.091 | RPLP0 | 1.104 | |
| B2M | 4.73 | B2M | 2.21 | RPLP0 | 1.53 | B2M | 2.136 | B2M | 1.545 | |
| HD | GAPDH | 1 | GAPDH | 1.04 | GAPDH | 0.17 | GAPDH | 0.149 | GAPDH HPRT1 | 0.298 |
| HPRT1 | 1.68 | HPRT1 | 1.1 | HPRT1 | 0.17 | HPRT1 | 0.149 | |||
| ACTB | 3 | ACTB | 1.1 | ACTB | 0.38 | ACTB | 0.151 | ACTB | 0.381 | |
| B2M | 4.23 | B2M | 2.14 | RPLP0 | 1.5 | B2M | 2.08 | B2M | 1.034 | |
| RPLP0 | 4.73 | RPLP0 | 2.29 | B2M | 1.6 | RPLP0 | 2.237 | RPLP0 | 1.535 | |
For BestKeeper and DeltaCt SD, for NormFinder SV, and for geNorm M values are shown; geomean value is the geometric mean. The lower the value, the higher the stability. NB: geNorm applets released a couple of best RGs.