Figure 4.

Subcutaneous ASCs dynamically disassemble and reassemble their FAs. (A) Schedule of the nocodazole washout assay. (B) ASCs were incubated for 5 h with 10 µM nocodazole followed by washout, where the microtubules (MTs) were allowed to regrow for 0, 30 and 75 min. Cells were stained for paxillin (green), p-FAK (red), and DNA (DAPI, blue). Representatives of FA disassembly/reassembly are shown. Scale: 15 µm. (C–E) Kinetics of FA disassembly during MT regrowth and FA reassembly after MT regrowth. Quantification of the mean fluorescence intensity of paxillin (C), p-FAK (D), and FA size (E) (270 FA per condition) is depicted. The results are based on three independent experiments and presented as scatter plots showing mean ± SEM. Unpaired Mann–Whitney U-test, ** p < 0.01, *** p < 0.001. a.u., arbitrary units.