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. 2019 Oct 18;8(10):1272. doi: 10.3390/cells8101272

Figure 4.

Figure 4

Consistent SHP2 expression enhance RTKs downstream signaling and chromatin stability. (A) Western blot of PI3K and p-AKT protein expression level was significantly enhanced in the presence of EGF in both COMBO and CONTROL groups. β-actin was used as a loading control for western blot. The bands were quantified using ImageJ software, and the differences are represented by histograms. (B) Relative mRNA expressions of mTOR and MAPK1 in blastocysts (n = 5 per each group). (C) Relative mRNA expressions of BAX and BCL2 in blastocysts (n = 5 per each group). (D) Immunofluorescent co-localization of H3K56ac with SHP2 in bovine blastocysts (n = 20 per each group). The experiments were repeated 3 times and the data are shown here as a mean ± S.E.M. N.S, not significant. * p < 0.05, ** p < 0.01, *** p < 0.001.