Skip to main content
. 2019 Sep 22;8(10):1126. doi: 10.3390/cells8101126

Figure 4.

Figure 4

(A) NeuN immunofluorescence (a1–a9) and F-J B histofluorescence staining (a1′–a9′) in the CA1s of the ND-fed group (left column), HFD-fed group (middle column), and HFD/RAPA-fed group (right column) at sham (a1–a3, a1′–a3′), 2 days (a4–a6, a4′–a6′), and 5 days (a7–a9, a7′–a9′) after 2-min TI. In the HFD 2-min TI group, a few NeuN+ CA1 pyramidal cells (a8, arrows) and many F-J B+ CA1 pyramidal cells (a8′, asterisk) were observed at 5 days post-ischemia. However, at this time, abundant NeuN+ CA1 pyramidal cells (a9, asterisk) and a few F-J B+ CA1 pyramidal cells (a9′, arrows) were observed in the HFD/RAPA 2-min TI group. SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum. Scale bar = 35 μm. (B,C) The mean numbers of NeuN+ (B) and F-J B+ (C) CA1 pyramidal cells (n = 7/group). The bars indicate the means ± SEMs. *** p < 0.001 versus each sham group; ### p < 0.001 versus HFD 2-min TI group.