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. 2019 Sep 22;8(10):1126. doi: 10.3390/cells8101126

Figure 5.

Figure 5

(A,B) DHE fluorescence staining (A) and HNE immunohistochemistry (B) in the CA1s of the ND-fed group (left columns), HFD-fed group (middle columns), and HFD/RAPA-fed group (right columns) at sham (a1–a3, b1–b3), 2 days (a4–a6, b4–b6), and 5 days (a7–a9, b7–b9) after 2-min TI. In the HFD/RAPA sham group, DHE fluorescence (a3) and HNE immunoreactivity (b3) in CA1 pyramidal cells (asterisks) were significantly deceased compared to the HFD/Vehicle sham group. In the HFD/RAPA 2-min TI groups at 2 days post-ischemia, DHE fluorescence (a6) and HNE immunoreactivity (b6) were significantly lower than the HFD 2-min TI group. Note that DHE fluorescence (a8) and HNE immunoreactivity (b8) in the HFD 2-min TI group at 5 days post-ischemia were very low due to death of CA1 pyramidal cells. Scale bar = 60 μm. (C,D) Quantitative analyses of DHE fluorescence (C) and HNE immunoreactivity (D) in CA1 pyramidal cells (n = 7/group). Relative ratios were calibrated as percentages, with the ND sham group designated as 100%. The bars indicate the means ± SEMs. * p < 0.05, ** p < 0.01; *** p < 0.001 cresyl violet each sham group, # p < 0.05, ## p < 0.01, ### p < 0.001 versus ND-fed group, † p < 0.05, †† p < 0.01, ††† p < 0.001 versus HFD-fed group.