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. 2019 Oct 11;8(10):1241. doi: 10.3390/cells8101241

Figure 1.

Figure 1

Perilipin 5 (PLIN5) was up-regulated in liver tissues of non-alcoholic fatty liver disease (NAFLD) mice. (A) Six-week-old C57/bl male mice were fed with a high-fat diet (HFD) or chow diet (CD) for 10 weeks. The mRNA levels of PLIN5 in liver tissues of mice fed with chow diet (CD) and HFD were detected by qRT-PCR. (B) The protein levels of PLIN5 in liver tissues of mice fed with chow diet (CD) and HFD were detected by Western Blot. (C) Six-week-old C57/bl male mice were fed with methionine-choline-deficient diet (MCDD) for 1 week, 2 weeks, 3 weeks, 4 weeks, 6 weeks, and 8 weeks, respectively. The mRNA levels of PLIN5 were detected by qRT-PCR. (D) The protein levels of PLIN5 were detected by Western Blot. (E) Immunohistochemistry analysis of liver tissues of mice fed with MCDD (1–8 weeks) and control mice (0 week). Scale bar, 20 μm. These experiments were performed in triplicate. * p < 0.05; and ** p < 0.01.