Figure 2.

Hydrogen peroxide or lipopolysaccharide (LPS) treatment enhanced the expression of PLIN5. Hydrogen peroxide and LPS were used to induce cellular oxidative stress. (A) mRNA level of PLIN5 in cells with H₂O₂ treatment or control (phosphate buffer saline, PBS). (B) Western Blot analysis of PLIN5 protein levels in cells with H₂O₂ treatment or control (PBS). (C) mRNA level of PLIN5 in cells with LPS treatment or control (PBS). (D) Western Blot analysis of PLIN5 protein levels in cells with LPS treatment or control (PBS). (E) H₂O₂ treatment did not change the localization of PLIN5. PLIN5 localization analysis of cells with 400 μM oleic acid medium or H₂O₂ treatment. Green, PLIN5-EGFP; red, lipid droplets; blue, nucleus. Bar, 10 μm. These experiments were performed in triplicate. * p < 0.05; and ** p < 0.01.