Figure 4.

PLIN5 promoted LD formation and contact with mitochondria. (A) HepG2 cells were transfected with PLIN5 expression vector or pcDNA3.1 (control) vector. Then, the cellular lipid droplets were stained with BODIPY493/503 and observed by a confocal microscope. (B) The counts of cellular LDs in A. (C) HepG2 cells were transfected with PLIN5 siRNAs or negative control siRNAs. Then, the cellular lipid droplets were stained with BODIPY493/503 and observed by a confocal microscope. (D) The counts of cellular LDs in C. (E) HepG2 cells were transfected with the mito-Dsred vector and PLIN5 expression vector or pcDNA3.1 (control) vector. Then, the cellular lipid droplets were stained with BODIPY493/503 and observed by a confocal microscope. (F) HepG2 cells were transfected with mito-Dsred vector and PLIN5 siRNAs or negative control siRNAs. Then, the cellular lipid droplets were stained with BODIPY493/503 and observed by a confocal microscope. (G) HepG2 cells were transfected with mito-Dsred vector and then treated with 200 μM H₂O₂. Then, the cellular lipid droplets were stained with BODIPY493/503 and observed by a confocal microscope. The fluorescence intensity along with the dotted line was performed to illustrate the contacts between LDs and mitochondria. (H) The ratio of contacts between LDs and mitochondria was analyzed. These experiments were performed in triplicate. *** p < 0.0001.