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. 2019 Oct 11;8(10):1241. doi: 10.3390/cells8101241

Figure 5.

Figure 5

PLIN5 regulated the expression levels of mitochondrial function-related genes and apoptosis rate. (A) mRNA levels of COX2, COX4, and CS in cells transfected with PLIN5 expression vector or control vector. (B) mRNA levels of COX2, COX4, and CS in cells transfected with PLIN5 siRNAs or control siRNAs. (C) mRNA levels of GPX1, GPX2, SOD1, SOD2, TXNRD1, CAT, and PRDX3 in cells transfected with PLIN5 expression vector or control vector. (D) mRNA levels of GPX1, GPX2, SOD1, SOD2, TXNRD1, CAT, and PRDX3 in cells transfected with PLIN5 siRNAs or control siRNAs. (E) Apoptosis rate of cells transfected with PLIN5 expression vector or control vector. (F) Cells transfected with PLIN5 expression vector or control vector were treated with 200 μM H2O2 for 12 h. Then, the apoptosis rate was analyzed. These experiments were performed in triplicate. GAPDH was used as the reference gene. * p < 0.05; and ** p < 0.01.