Figure 6.

The expression of PLIN5 was regulated by the JNK-p38-ATF pathway. (A) Protein levels of ATF1, ATF4, p-p38, p38, p-JNK, and JNK were detected by Western Blot. (B) The gray value analysis of A. (C) mRNA levels of ATF1, ATF3, and ATF4 in cells with 200 μM H₂O₂ treatment were detected by qPCR. (D) The cells were transfected with ATF1 expression vector or pcDNA3.1 vector. The protein levels of ATF1 and PLIN5 were detected through Western Blot. (E) The cells were transfected with ATF4 expression vector or pcDNA3.1 vector. The protein levels of ATF4 and PLIN5 were detected through Western Blot. (F) The effects of ATFs’ expression on PLIN5 transcriptional activity were detected by dual-luciferase reporter assay. (G) Protein levels of p-ASK1, Ask1, p-p38, p38, p-JNK, JNK, and PLIN5 were detected by Western Blot. (H) The gray value analysis of G. GAPDH was used as the reference protein. These experiments were performed in triplicate. * p < 0.05; ** p < 0.01; and n. s., not significant.