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. 2019 Oct 29;10:1060. doi: 10.3389/fgene.2019.01060

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Copyright © 2019 Wang, Han, Liu, Khan, Xiaerbati, Yu, Huang and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative mRNA and protein expression analysis of the TTI2 mutations. (A) Quantitative RT-PCR (qRT-PCR) analysis of TTI2 mRNA in peripheral blood leukocytes from patients (II-1 and II-3), unaffected parents (I-1 and I-2), and a control individual (C1). The results of the quantification of TTI2 transcripts were first normalized to the ACTB housekeeping gene. The values presented are the means of triplicate determinations ± standard deviation (SD). The C1 was set to 1.0. (B) TTI1, TELO2, and TTI2 protein expression in lymphocytes obtained from the family members and C1, with β-actin serving as a loading control. The results represent one of three similar experiments.