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. 2019 Oct 29;10:1242. doi: 10.3389/fphar.2019.01242

Figure 1.

Figure 1

ESM-HDAC528 reduces pro-inflammatory cytokine production both in vitro and in vivo. (A) Cytokine production by BMDMs from CES1/Es1elo mice and WT mice after stimulation with LPS (10 ng/ml) or LPS (10 ng/ml) + IFN-γ (10 U/ml) in the presence of increasing concentrations of ESM-HDAC528 for 24 h. n = 3. (B) Design of acute thioglycolate model. Transgenic mice were treated for 4 days with daily i.p. injection of 3 mg/kg ESM-HDAC528 (n = 6) or vehicle (n = 6), on day 4, 24 h after i.p. injection PECs were isolated. (C) Total number of cells isolated from the peritoneal lavage in each group n = 6 per group. (D) Cytokine production by PEMs isolated from the mice (n = 6) of each group attached and then stimulated for 24 h with LPS (10 ng/ml) or LPS (10 ng/ml) + IFN-γ (10 U/ml). Statistical significance was determined by unpaired t-test (C) or two-way ANOVA with Bonferroni correction (A, D) (p < 0.05). All error bars represent the SEM. ns = p value > 0.05, *p value ≤ 0.05, **p value ≤ 0.01, ***p value ≤ 0.001.