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. 2019 Oct 29;10:1242. doi: 10.3389/fphar.2019.01242

Figure 5.

Figure 5

ESM-HDAC528 modulates PEM maturation and activation to a lesser extent in an atherosclerosis model. (A) Percentage of mature (CD11b+ and F4/80+) macrophages from freshly isolated PECs 24 h after ESM-HDAC528 injection and of cells expressing Ly6C and CD64 within this population. n = 5 per group. (B) MFI of F4/80 and CD11b in the mature macrophages 24 h after injection. n = 5 per group. (C) General gating strategy for activation marker expression on PEMs after attachment and 24 h stimulation for activation. Antibodies were conjugated to either APC or PE dependent on the panel. (D) MFI of the positive peaks for pro-inflammatory surface markers in PEMs Isolated, attached and stimulated for 24 h with LPS (10 ng/ml) or LPS (10 ng/ml) + IFN-γ (10 U/ml). n = 5 per group. (E) MFI of the positive peak for alternative activation surface markers in PEMs attached and stimulated for 24 h with IL-4 (20 ng/ml). n = 5 per group. (F) Cytokine production by PEMs isolated from the mice (n = 5) of each group stimulated 24 h with LPS (10ng/ml) or LPS (10ng/ml) + IFN-γ (10 U/ml). Statistical significance was determined by unpaired t-test (A and B) or two-way ANOVA with Bonferroni correction (D–F) (p < 0.05). All error bars represent the SEM. ns = p value > 0.05, *p value ≤ 0.05, **p value ≤ 0.01, ***p value ≤ 0.001.