Table 1. Primer sequences and amplification size used for RT-PCR.
Gene | Primer sequences (5′–3′) | Amplification size (bp) |
---|---|---|
RQ-TRAP TS | AATCCGTCGGAGCAGAGTT | |
RQ-TRAP ACX | GCGCGGCTTACCCTTACCCTTACCCTAACC | |
NANOG | AGAAGGCCTCAGCACCTAC GGCCTGATTGTTCCAGGATT |
205 |
OCT4 | CGACCATCTGCCGCTTTGAG CCCCCTGTCCCCCATTCCTA |
577 |
SOX-2 | CCCCCGGCGGCAATAGCA TCGGCGCCGGGGAGATACAT |
448 |
GAPDH | GAAGGTGAAGGTCGGAGTC GAAGATGGTGATGGGATTTC |
228 |