Figure 2.

Structures of the three model systems from grids prepared on the TrEM setup. To test our ability to make high-quality EM grids by spraying proteins on a fast-moving plunging grid, three samples were tested and data are shown with representative micrographs and 3D reconstructions: (a, d) E. coli ribosome (0.72 µM in 50 mM HEPES pH 7.5, 100 mM KAc, 8 mM MgAc₂), (b, e) apoferritin [30 µM (24-mer) in 20 mM HEPES pH 7.5, 150 mM NaCl], and (c, f) porcine thin filaments [5 µM (actin monomer) in 10 mM MOPS pH 7, 50 mM KAc, 3 mM MgCl₂, 1 mM EGTA]. The scale bars in (a), (b) and (c) represents 50 nm. The scale bars in (d), (e) and (f) represents 5 nm.