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. 2019 Oct 6;8(10):1208. doi: 10.3390/cells8101208

Figure 2.

Figure 2

(A) Schematic of lentiviral construct pHAGE-NFkB-TA-LUC-UBC-dTomato. (B) Effects of treatment with 10 ng/mL TNFα, 1 μM senexin B or their combination on luciferase expression in the indicated pooled populations or clones of wild-type (WT) and double knockout (dKO) cells. Data are presented as mean ± SEM (n = 3). Asterisks: p < 0.01 for the difference between TNFα and TNFα + senexin B readouts. (C) Effects of different concentrations of senexin B on luciferase expression in the indicated WT and dKO 293 clones treated with 10 ng/mL TNFα for 3 h. % control (Y axis) was calculated relative to cells without the inhibitor. (D–F) Effects of different concentrations of dCA, TPCK and bortezomib on luciferase expression in 293-WT-NFKB-LUC#8 and 293-dKO-NFKB-LUC#2 reporter clones treated with 10 ng/mL TNFα for 3 h.