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PR-619 induced G0/G1 arrest in human UC cells. (A) T24 and BFTC-905 cells were treated with PR-619 (7.5 μM) or DMSO for 24 h. Cell cycle analyses were performed through flow cytometry with PI staining. (B) Quantitative data are presented. (C) Cells were treated with PR-619 (5, 7.5, and 10 μM) or DMSO for 24 h. Total cell lysates were assessed for p21 using Western blot analysis. Results shown are representative of at least three independent experiments.
