Figure 6.

CPT1A expression modulates histone acetylation in an androgen-dependent manner. (A): Representative western blot of cell lysates with pan-acetylated lysine antibody. (B): Western blot analysis of acetylated histone lysine residues from C4-2 cells (KD and OE) cultured in FBS or CSS (steroid- free media). (C): Lysine acetylation of histones H3 and H2B, isolated from C4-2 cells cultured in CSS, with or without p300 inhibitor A485 (5 µM), and measured by mass spectrometric analysis. Additionally, cells were treated with C¹³-acetate for 24 h in CSS with or without A485, followed by histone isolation and mass spectrometric analysis. Numbers on bars indicate amount of specific 13C-acetylated histone peptides identified.