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. 2019 Nov 1;11:9339–9351. doi: 10.2147/CMAR.S229441

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© 2019 Tian et al.

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The overexpression of CLDN12 significantly promoted the malignant phenotype of fetal-osteoblast line cell. (A) Western blotting was utilized to examine the expression of CLDN12 and the activities level of the Akt protein. (B) The expression location of CLDN12 was detected via immunofluorescence. The CLDN12 protein was stained with red color and the nuclear was stained with blue color. (C) Growth curve of hFOB.1.19 cells detected by the CCK-8 assay. (D) The abilities of hFOB.1.19 cells to form colonies under 2D culture conditions were determined via the colony formation assay. (E) The wound-healing assay was utilized to explore the migration ability of hFOB.1.19 cells in vitro. (F) The Transwell chambers method was utilized to explore the impact of CLDN12 overexpression on the migratory ability of hFOB.1.19 cells in vitro. **P<0.01, compared with the vector group.

Abbreviations: CLDN12, claudin-12; CCK-8, Cell Counting Kit-8.